Since the temporal pattern of SWS and quiet-awake episodes and their onset and duration varied among rats, the analysis was performed for mean values over 60-min time to minimize this variability.
In cells derived from young rats, the analysis resulted in 3318 differentially expressed PS, 1604 increasing (YI) and 1714 decreasing (YD).
Because of the presence of sequences from other microbial genomes and the rat host, the analysis and compression to a unigene set was necessarily an iterative process.
Because overall distinctness is more subtle in wildtype rats than in cLH/cNLH rats, limiting the analysis to those trials that guarantee high discriminative power, may thus even improve the identification of different subgroups.
Left ventricle homogenates were prepared from rats for the analysis of protein levels.
The large number of rats in the analysis (192) provided a unique statistical power to identify novel genes that exhibit gender-selective gene expression and can be used for further elucidation of regulatory control elements, some of which may depend on tissue context.
One limitation of this study was the small amount of CSF obtained from a single rat, which prevented the analysis of individual animals to determine the degree of inter-individual variability in the proteins levels following injury.
BM participated in the experiments in rat tissues and the analysis of data.
To measure the nociception associated with the development of arthritis in AIA rats, the gait analysis was employed in our study.
Compared to the three different doses (high, medium, and low) of KJA with normal saline control rats, the microarray analysis identified 1991, 2163, and 1677 differentially expressed genes in the rat respectively, of which 444 genes were raised and 670 genes were decrease spliced together in the three doses tested.
