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Any rat that failed to find the platform within the allotted time was guided to its location by the experimenter and allowed to remain on the platform for 30 s before commencing the next trial.
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Rats that failed to run in a regular way on the treadmill (contact of the forelimbs with the front wall of the treadmill, frequent immobility or gallop) at the end of the selection period were excluded.
The accommodation lasted for three days, and rats that failed to run the minimum amount of distance of the treadmill (600 m) and in the wheel (600 m) daily were excluded.
The distinction between the groups becomes evident when analysis is focused on the degree of motor function (Fig. 7e h), i.e., by omitting the rats that failed to make any steps (those aligned in parallel to the x-axis in Fig. 7a d).
Rats that failed to respond within the first 5 min were presented with a noncontingent cue.
Rats that failed to locate the platform within 90 s were guided to the platform manually.
Rats that failed to reach the criterion to be tested under a FCN8 schedule after 20 training sessions were excluded.
Rats that failed to show SCM CPP (defeats, n = 4, controls, n = 4) were not included in any of the analyses assessing SCM consumption and CPP [ 20, 47].
Rats that failed to walk in a regular manner on the treadmill (contact of the forelimbs with the front wall of the treadmill, frequent immobility or galloping) at the end of the selection period were excluded from further experiments.
The results indicate that activated kinases such as pERK 1/2, p-p38 MAPK, but not pJNK, are upregulated in injured rats that develop CNP as compared to injured rats that fail to develop CNP.
In either case, the present data suggest that neuronal activity in the IL is altered in rats that fail to extinguish fear relative to those that extinguish normally.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com