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The primers were designed with (http://www.ncbi.nlm.nih.gov/tools/primer-blast/). Human β-actin and rat β-actin were used to normalize human and rat templates, respectively.
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These images were normalized using F-FDG rat brain templates.
The L-Snord115 expression constructs were generated via polymerase chain reaction (PCR) amplification of rat genomic templates.
As previously done in earlier Bubbles studies [197,201,202], Vermaercke and Op de Beeck also compared rat behavioral template to the "optimal" template obtained by simulating an ideal observer.
To verify the anatomical location of the signal, PET images were co-registered to the anatomical data of a MRI rat brain template.
Time-averaged tissue radioactivity images were manually co-registered to a standard rat histological template [19] using seven degrees of freedom (rigid body transformation plus one scaling constant).
(ii) A second set of VOIs was automatically generated in the cortex, striatum, hippocampus, thalamus, and cerebellum by using the regions proposed by the PMOD rat brain template.
[18F]NaF scans were co-registered with a standard magnetic resonance (MR) rat brain template to delineate regions of interest (ROIs); this method was previously described and validated with [11C]AF150(S) in rats [42].
Whole brain VOIs were manually drawn in both the entire ipsilateral and contralateral hemispheres containing the territory irrigated by the middle cerebral artery on slices of a MRI (T2W) rat brain template from the PMOD software.
Each intracranial individual image was spatially normalised with the intracranial FDG-PET rat brain template in SPM8.
All brain PET images were spatially normalized to the FDG rat brain template (Schiffer et al., 2006) (PMOD 3.4, PMOD group, Zurich, Switzerland) using nonlinear registration on Statistical Parametric Mapping (SPM8, University College of London, London, UK).
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