Sentence examples for rat stem cells from inspiring English sources
In a 2010 Cell paper, Nakauchi's team described growing rat pancreases, using rat stem cells, in apancreatic mouse embryos.
McKay and colleague Robert Tsai, also of NINDS, first found the gene in cultured rat stem cells.
CD105, a polyclonal rabbit antibody (Santa Cruz Biotechnology, Inc., Dallas, TX, USA), was used as a marker for rat stem cells.
Mutant mice are traditionally crafted by disabling genes in embryonic stem cells, but no one could get rat stem cells with incapacitated genes to survive in the laboratory.
To see what power nucleostemin wields, the researchers looked at its effects in both rat stem cells and human cancer cells.
This result is consistent with the previous study, which shows that TRPC5 predominantly expresses in the CNS and is involved in proliferation of embryonic rat stem cells [23].
Briefly, LDBM cells were cultured for 2 days in Iscove's Modified Dulbecco's Medium (Invitrogen) supplemented with 10% fetal calf serum (FCS, HyClone, Logan, UT), 2% penicillin and streptomycin (P/S), and 100 ng/ml each of recombinant rat stem cell factor (SCF), megakaryocyte growth and development factor (MGDF) and granulocyte colony-stimulating factor (G-CSF) (all from Amgen, Thousand Oaks, CA).
Uterine-like tissues were then regenerated and maintained in vitro for up to 10 d through decellularized uterine matrix (DUM) reseeding with adult and neonatal rat uterine cells and rat mesenchymal stem cells followed by aortic perfusion in a bioreactor.
Here, we show that spheroids that contain rat neural stem cells (NSCs) or rat glioma stem cells (cancer stem cells, CSCs) express isoforms 1 and 2 of pyruvate kinase (PKM1 and PKM2); however, the expression of PKM2 is considerably higher in glioma spheroids.
Tong and colleagues created a TP53-deficient Dark Agouti rat (referred to subsequently as the TP53-deficient DA rat) via homologous recombination in the rat embryonic stem cells (Tong et al., 2010).
This demonstrates that careful application of standard culture methods enables reliable rat transgenesis despite the fastidiousness of rat pluripotent stem cells.
