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It shows unexpectedly large stores of GS in epidermal keratinocytes in human and rat skin sections.
To explore this, GS localization was investigated using immunohistochemistry and double-labeling of young and adult human and rat skin sections as well as skin cells in culture.
Unfixed 7 μm cryostat human and rat skin sections, cultured human keratinocytes and fibroblasts, as well as cultured rat astroglial cells were prepared as described previously [91].
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To determine whether GIRK1 and -2 are transported to the peripheral terminals of sensory neurons, we carried out immunostaining of rat and human skin sections.
Immunofluorescent studies in frozen sections of human and rat skin utilizing a Wilson protein antibody demonstrated abundant expression of the Wilson protein in the secretory and ductular epithelial cells of the sweat glands both in human and rat.
Skin sections from male Wistar rats were immunofluorescently stained as follows.
The occurrence of different intercellular junctions in epithelial rat skin tumours induced by methylcholanthrene was investigated using thin sections and freeze-fracture replicas examined by electron microscopy.
Immunohistochemical staining of skin sections from glabrous and adjacent hairy skin of adult rat hindpaw showed that mTOR and phospho-mTOR were extensively expressed in subsets of primary afferent sensory fibers, identified from co-staining with PGP, a general marker for sensory afferents, as well as in non-neuronal cells of surrounding dermal tissue (Fig. 1A, B).
The patches were devoid of hypersensitivity reactions on rat skin.
Skin permeation of PCL was studied using the rat skin.
The rat skin without any treatment was used as the control.
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