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The application of this sensor in the analysis of zinc ions in the rat serum samples was also evaluated.
RESULTS: (1) Increased absorption was found in 43percentt of rat serum samples.
Rat serum samples analysed for MOG reactivity were also assayed for expression of circulating CRT.
aCO4-MMP = 1438'GTPSVDHSFL'1447; HS(1 or 2) = human serum samples; RS(1 or 2) = rat serum samples.
The pure compounds, rat serum samples, and total glycolipids from brain or spinal extracts were dissolved in MeOH and analyzed by mass spectrometry (MS).
Rat serum samples (diluted 1 50 in PBS containing 0.05% Tween and 1% BSA) were added in duplicate and incubated for 1 hour in a 37°C humid chamber.
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To each 100 µl of rat serum sample, 100 µl of 0.1N sodium acetate (pH = 5.0) and 100 µl of β-glucuronidase enzyme (5000 units/ml, type HP-2 from Helix Pomatia) were added and vortexed for 5 seconds.
CSF samples were centrifuged and the supernatants were diluted 1 5 in Bio-Plex rat serum sample diluent and mixed with antibody-coated microspheres on a membrane microtiter plate.
Measure 7 biomarkers per microplate well from 6 group rat's serum samples displayed the concentration profiles by quantitative chemiluminescence.
A third set of three samples was generated by spiking commercially available rat serum; one sample contained no added BPA, one was spiked with BPA-G, and one was spiked with both uBPA and BPA-G.
Unless specified otherwise, a series of threefold dilutions of different mouse, cotton rat, or human serum samples, starting with a 1/100 dilution, were loaded on the coated plates.
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