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One hundred μl rat sera was mixed with 100 μl 10% trichloracetic acid (TCA, Sigma Aldrich) and allowed to sit on ice for 15 minutes in the dark before being centrifuged for 5 minutes at 4°C and 16,100 g.

Rat sera were diluted with PBS at 1 20 and added in duplicate wells.

The contents of Collagen I in rat sera were determined with ELISA.

Oxidant and antioxidant parameters in liver tissue and AST, ALT, and LDH levels in rat sera were measured in all groups.

Rat sera were diluted 1 200 in PBS containing 1% bovine serum albumin (BSA) as blocking agent, and 0.2 mL was added to each well in triplicate.

When IMR-90 cells grown in the presence of rat sera were analyzed, we found that at PDL 37, the percentage of SA β-gal-positive fibroblasts cultured in DMEM containing 10 % CR serum was reduced more than 8 folds compared to that of cells of the same passage number grown in 10% AL serum (Fig. 2C).

Both the sera and the polyclonal antibody were found to react to cultured rat cortical neurons and the reactivity of sera was reduced by incubation with recombinant INA.

IGF-1 concentration in blood sera was determined using IGF-1 (Mouse/Rat) ELISA kit (ALPCO Diagnostics, Salem, NH) according to manufacture's protocol.

Goat anti-rat IgG-FITC, rat serum and hamster sera were from Sigma, Inc. Saint Louiss, MO, USA).

Fetal calf, horse, rat, human and rabbit sera were supplied by Aleken Biologicals.

A neutralizing anti-IL-6 Ab (Clone MP5 20F3) or rat IgG (purified from sera) were generated in-house and 200 μg injected i.p. on days 0, 2, 4, and 6 postinfection, with cells harvested on day 7.