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Rather than firing a second time when the rat reached the same place on its return trip, the cells fired when the rat was two steps away from the opposite end of the track, the authors report online today in Science.
When the rat reached the platform, it sat for 30 s before being removed.
Testing at each stage continued until the rat reached the criterion of six consecutive correct trials.
An arm entry was recorded when the rat reached the food cup at the end of an arm.
An arm entry was recorded when the rat reached the terminal platform at the end of the arm.
After the rat reached the terminal platform and consumed the milk, it was immediately returned to the start box for the beginning of the choice run.
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All control rats reached the learning criterion on both the inbound and outbound components of the W-track continuous alternation task within a few days.
After the rats reached the operant conditioning inclusion criteria of 80% on each side, neural data was recorded for six sessions.
Importantly, the levels of IL-17 in supernatants from pMOG-IFNbeta-treated rats reached the same levels as in supernatants from pCI-treated controls after 48 h and 72 h restimulation, and were significantly higher than in supernatants from pMOG-treated rats after 48 h restimulation (p<0.05) (Fig. 7A).
Altogether 50 rats reached the humane endpoint in the course of the experiment and were killed.
Behavioral testing began after 2 months of treatment, when rats reached the age of 3.5 months.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com