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Each rat of Group 3 received 0.1 μg AfB1 per gram weight throughout the experiment.
For microarray analysis, 1 μg RNA from each rat of Group 1 was combined and 1 μg RNA from each rat of Group 2 was combined.
95 of 96 rats survived over the time course of the study; one rat of group B was died during anesthesia, and severe infection was noted in one rat in group B. Therefore, two additional rats were operated on to maintain the experimental design numbers (total number of rats, 98).
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Rats of Group II or Group V were injected with NaCl (1 M) at an analogous dose and frequency.
Every rat of groups 3 and 4 received the amount of the liquid proportional to its body mass.
The ratio of mean EMI volumes in control and experimental rats of Group 1 was 14 1.
As shown in Table 1, S. muris-infected rats of group (GII) had significant reduction in their body weights when compared with the uninfected control group (P = 0.0004).
The histopathological analysis of EMI samples from experimental rats of Group 2 demonstrated complete disappearance of endometrial cysts suggesting a cytotoxic effect of DNIC on the tumours.
In the current study, rats of group II (high dose) showed a significant increase in their body weights compared to the control group (Group I) in the first week of the study.
Rats of Group 2 presented alterations corresponding to a diabetic state in almost all the parameters measured, none of the animals showed a tendency to improve spontaneously, two of the rats died at 66 and 72 days.
Further, the animal in group A and B received 2 immunizations with bovine collagen type II (CII), and rats of group C served as controls.
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