Sentence examples for rat marker from inspiring English sources
For example, using Liftover, base pair positions for rat marker D10Mgh12 are not homologous to the mouse genome.
The rat marker names were listed in chromosome and base-pair order in an Excel spreadsheet, along with the rat genetic position, the homologous mouse position, and the mouse genetic position.
Using this method, all but 10 of the 138 markers were converted; the remaining markers were positioned in Ensembl, and using Ensembl's Comparative Genomics tool, genes adjacent to the rat marker and both homologous and contiguous to the mouse genome were determined.
The mouse position of the nearest homologous gene in a contiguous sequence of genes was used as the rat marker's homologous position.
Using the above methods, all but three rat markers— D2Wox32, D8Mit6 and D10Mgh2 were aligned to the mouse genome.
The homologous mouse positions of the rat markers were interpolated to the Revised Shifman genetic map of the mouse (Cox et al, 2009) using the Center for Genome Dynamic's online Mouse Map Converter (http://cgd.jax.org/mousemapconverter/).org/mousemapconverter/
Rat genome genetic positions for the rat markers were determined by interpolation to the SNP map recently published by the STAR Consortium (Saar et al, 2008); the version 3.4 base-pair positions and STAR map cM positions are publicly available online at http://www.well.ox.ac.uk/ rat_mapping_resources/ SNPmaps.html.html
For example, a rat QTL marker was excluded if it was within a rat QTL but outside of a homologous mouse QTL.
Like the rat NP marker KRT19, KRT18 has also been identified in human notochordal cells during development and also in the human NP.
Our results are in accordance with previous literature showing that CD68 is largely used as human and rat monocyte marker, but CD163 expressed by human blood monocytes appears restricted to M2 macrophages in rat [ 25- 27].
AAA tissue sections were incubated (30 min at 40 °C) with the following antibodies: alpha-actin (Abcam cat 5694 rabbit, 1.4 μg/mL), Ly-6G/-6C (NIMP-R14 rat, neutrophil marker) (Abcam, cat#2557 rat, 5 μg/mL), striatin (index of E2 receptor signaling; EMD Millipore, cat AB5779 rabbit, 5 μg/mL), or ER-TR7 (fibroblast marker; Abcam cat 51824 rat, 2 μg/mL).
