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Purpose: The purpose of this study was to explore regional differences in radiosensitivity of rat lung using lung function and computed tomography (CT) density as endpoints.
We have developed a hyperpolarized 129Xe experiment system, which could yield enough amount and polarization for dynamic analysis in a rat lung using the 3-T MRI system.
Total RNA was isolated from the rat lung using TRIzol® reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacturer's instructions.
We isolated nucleic acid from rat lung using Qiagen Genomic-tips, essentially as described by the manufacturer, with the following modifications.
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Bhaskaran et al performed miRNA profiling at 7 time points in rat lung development using an in-house platform and identified 21 miRNAs in 4 clusters that showed significant changes in expression [20].
This effort was to generate gene expression profiles for rat lung tissue using high-throughput microarray technologies to distinguish levels of injury based on the differential expression of specific groups of genes thought to be involved in this process.
We designed this study to evaluate the effect of NO on the morphogenesis of hypoplastic lung using nitrofen-induced rat lung explant model.
In the present work the nanospheres suspension is supposed to be administered into the rat lungs by using a micro-sprayer device which renders the suspension into micro-droplets capable to reach the deepest parts of pulmonary tract.
As many intrapulmonary arteries as possible were dissected from rat lungs and used for the extraction of total RNA with an RNeasy Micro Kit (Qiagen).
Nuclear extracts were performed on homogenized rat lung tissue by using a NE-PER NuCytoplasmicytoplasmic Extraction Kit (Fisher Scientific Ireland, Dublin, Ireland), and NF-κB (p65) measured by using an NF-κB Transcription Factor Assay Kit (Cayman Chemical Company, Ann Arbor, MI, USA).
To isolate AEC II, rat lung was perfused using solution II (0.9% NaCl, 0.1% glucose, 30 mM HEPES, 6 mM KCl, 0.1 mg/ml streptomycin sulfate, 0.07 mg/ml penicillin G, 0.07 mg/ml EGTA, 3 mM Na2HPO4, and 3 mM NaH2PO4, pH 7.4) to clear the red blood cells.
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