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Rat FRβ cDNA was prepared from a product derived from Lewis rat liver using (RT-PCR).
OPH was semi-purified from 100 g of rat liver using the method described by Tsunasawa [ 20] with the following modifications.
This finding is consistent with a study to predict the levels of necrosis in the rat liver using this same compendium of hepatotoxicants [ 24].
Immunostaining of sections from a normal rat liver using a monoclonal antibody against rat PECAM-1 revealed positivity along the sinusoids in a similar pattern to ICAM-1, suggesting PECAM-1 positivity of SECs.
An initiation/promotion bioassay for chemical carcinogens and tumor promoters has been developed in rat liver using presumed preneoplastic lesions, foci of gamma-glutamyltranspeptidase (GGTase -positive hepatocytes, as the endpoint.
Finally, we measured the activities of different HDAC isotypes present in partially purified extract from rat liver using the fluorescent HDAC substrates MAL (unselective), B61 (selective for HDAC1, and to some extent also converted by HDAC3) and B12 (selective for HDAC6).
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Total RNA was extracted from 100 mg rat livers using TRIzol reagent.
For qPCR studies, total RNA was extracted from the rat livers using RNAiso Plus reagent (TaKaRa Biotechnology Co., Dalian, China).
To test the quality of the "blood vessels" produced, the researchers cultured a chunk of tissue made from rat liver cells using their technique.
However, the present results demonstrated that MC-I activity in the rat liver measured using 18F-BCPP-BF was impaired by acetaminophen, even at a low dose of 100 mg/kg, a dose at which no apparent effects on hepatic function have been indicated in previous studies [24, 31, 32].
Total RNA was isolated from rat liver samples using Trizol reagent, followed by purification with RNeasy Mini Kit (Qiagen, MD).
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