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The protection afforded by EPO against renal IRI also might be partly due to its antioxidant effects, because EPO administration before the onset of renal ischaemia has been shown to induce a significant decrease in lipid peroxidation and an increase in the activity of endogenous antioxidant defense mechanisms, such as SOD and GSH, in rat kidney tissue [42].
Since SCX and ERLIC have both been used for fractionation of both modified and unmodified peptides to some extent, they were compared here in detail for the analysis of tryptic peptides of rat kidney tissue.
As shown in Table 2, proteins identified from rat kidney tissue in the ERLIC04 method are involved in various biological processes such as protein metabolism, RNA metabolism, cellular component organization, transport and developmental processes.
We describe here a method for concurrent analysis of phosphopeptides, glycopeptides and unmodified peptides in a tryptic digest of rat kidney tissue with a sequence of ERLIC and RP-LC-MS/MS in a single experimental run, thereby avoiding inter-experimental variation.
Two SCX and four ERLIC gradients were compared in details, and one ERLIC gradient was found to perform the best, which identified 2929 proteins, 583 phosphorylation sites in 338 phosphoproteins and 722 N-glycosylation sites in 387 glycoproteins from rat kidney tissue.
We used normal rat kidney tissue, which is a suitable model for carcinogenesis due to oxidative stress.
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We designed this study to identify changes in HMGB1 expression in rat kidney tissues after ischemia reperfusion injury and effects of EP on the expression of HMGB1.
A coarse speckled cytoplasmic staining of mitochondria HEp-2 cells was read as AMA positive, but AMA was then further confirmed by IIF on rat kidney tissues (Euroimmun).
Among the factors involved in kidney development and kidney injury recovery, hepatocyte growth factor (HGF) and bone morphogenetic protein-7 (BMP-7) were analyzed and both found significantly increased by icariin in CKD rat kidney tissues.
For determination of gene expression in rat kidneys, tissue stored at −80°C was homogenized (Polytron, Kinematica Gmbh, Littau, Switzerland).
Basile et al. [ 14] showed that in rats kidney tissue MMP-2 activity increased two- to threefold in days 1 to 3 after ischemic acute renal failure and MMP-9 activity increased two- to threefold in days 2 to 3. To our knowledge, MMP activities in urine have not been measured in equine patients.
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