Each determination was made for each flexor digitorum tendon at the level of the wrist, at three microscope field locations per rat, in two to three separate sections per rat.
The purpose of the validation program was to demonstrate the performance of two versions of the uterotrophic bioassay, the immature female rat and the adult ovariectomized rat, in four standardized protocols.
Pre-eye-opening data were obtained on P11 in one rat, P12 in three rats, P13 in six rats, P14 in eight rats, and P15 in one rat; post-eye-opening data were collected on P14 in one rat, P15 in eight rats, and P16 in eight rats.
Materials and methods: Sixty-five male Wistar rats in three groups: I (n=25) controls, II (n=20) sham operation, III (n=20) occlusion of the hepatoduodenal ligament (Pm).
After fasting overnight, the impaired glucose tolerance of rats in three groups were measured by OGTT (Figure 3).
In group IV, no macroscopic tumor were found in 2 rats, in three rats only locally limited metastases were found.
Experiments were performed with cells from three female rats in three individual experiments, and with at least three parallel wells for each concentration of the drugs.
In all three groups, the white blood cell count was lower than 100/mm when examined using an optic microscope, indicating that no rats in three groups developed peritonitis.
Rats in three treatment groups were intragastrically administered with different concentrations of TBK (dissolved in 0.5% sodium carboxymethylcellulose) at 1 ml/100 g once a day for 4 weeks.
Treatment with NT-3 + NR2d induced the appearance of larger responses (peak amplitude 1.9 ± 0.7 mV; Fig. 2E) but did so in only a few rats (in three rats out of 10 studied).
