We could record an ex vivo proliferative T cell response to RSA as well as to Cit-RSA in rats immunised with modified protein and also in rats immunised with the unmodified protein, as demonstrated by stimulation index values over 1.0.
Inguinal lymph nodes from Lew.1AV1 rats immunised with RSA and Cit-RSA were removed 10 days after immunisation; single-cell suspensions were prepared and resuspended in Dulbecco's modified Eagle's medium supplemented with glutamine, penicillin, streptomycin, HEPES and 10% fetal calf serum (Life Technologies, Paisley, Renfrewshire, UK).
Patients' autoantibodies cross-reacted with FimH, and rats immunised with FimH developed anti-FimH antibodies that cross-reacted with human and rat LAMP-2.
For comparison, LNC of WKY rats immunised with Mtb were harvested at the time points corresponding to different phases of AA in LEW rats.
Three representative sera obtained from rats immunised with tau peptide were able to recognise NFTs and neuropil threads in the entorhinal cortex of AD brains.
However, the fact that Kim and colleagues did not observe reduced T cell responses in TNFα treated rats immunised with a control antigen (hen egg lysozyme) would argue against a generalised immuno-suppressive effect [ 1].
Individual serum samples from Lew.1AV1 rats immunised with RSA and Cit-RSA were obtained at different time points, namely 12, 24 and 35 days after immunisation (by tail bleeding) as well as at 61 days after immunisation (by heart puncture).
Brain sections were incubated for 20 minutes at RT in 0.01 M of PBS, pH 7.4, containing 0.3% Triton X-100 and 1% H2O2, followed by a 30-minute incubation in the blocking solution (0.01 M of PBS, containing 0.3% Triton X-100, 1% horse serum), followed by overnight incubation with sera from transgenic rats immunised with the tau peptide vaccine (diluted 1 1,000) at 4°C.
Lew.1AV1 rats were immunised with the non-immunogenic autoantigen RSA or the modified counterpart Cit-RS Aand the differences in induced immune responses were analysed in vitro.
