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In the genomic analysis, two RGD customized genome browsers, the Rat Genome Browser (http://rgd.mcw.edu/fgb2/gbrowse/rgd_904/) and the Human Genome Browser (http://rgd.mcw.edu/fgb2/gbrowse/human_36_3/), were accessed from the 'Genome Tools' icon on the RGD home page (http://rgd.mcw.edu/).mcw.edu/
These two rat syntenic blocks house 93 cardiovascular and obesity disease genes [data accessed January 2013, from the Rat Genome Browser (http://rgd.mcw.edu/fgb2/gbrowse/rgd_904/)].
List of non-synonymous mutations derived from Nimblegen array target sequencing and bounded by the genes Myo1c and Slc6a4 and using the flanking markers D10Rat161 and D10Rat238 [62685555-67091740]. *Position location in base pairs (bp) was determined using the Rat Genome Browser Gateway using the Baylor 3.4/ build rn4 (http://genome.ucsc.edu/cgi-bin/hgGateway?org=rat).
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(a) To study the localization of piRNAs within various repeat families, we mapped the genomic coordinates of the piRNAs (obtained from BLAST) with the chromosomal positions corresponding to the repetitive regions in human, mouse and rat genome downloaded from UCSC genome browser [ 32].
Chromosomal positions corresponding to genes, introns, CDS, 5/UTR, 3/UTR, for human, mouse and rat genome were downloaded from UCSC genome browser [ 32].
Maps of the mouse compared to dog, rat, and human were retrieved from the Ensembl genome browser (Comparative Genomics - Synteny, http://www.ensembl.org/Mus_musculus/Location/Synteny) database.
Rat promoter sequences were obtained by blatting the 2.5-kb mouse promoter sequences into the UCSC genome browser of rat.
The mapping of the differentially expressed genes to QTLs for arthritis was investigated using Rat and Human Genome browsers from Ensembl, Rat Genome Database and the ARB Rat Genetic Database.
Visual inspection of the conserved sequences among the human, chimpanzee, mouse, rat, and chicken genomes (UCSC genome browser hg16 build, table mxPt1 Mm3RnGg_pHMM) along the regions covered by our array suggested a correlation of origin peaks with the position of conserved elements.
We downloaded the entire set of human, mouse and rat syntenic regions from UCSC genome browser and mapped the piRNA clusters which are conserved.
The genome annotations were retrieved from ENSEMBL's biomart server (rat build 70) or the UCSC genome browser (human) and we used custom scripts to extract the coordinates of promoters (which we defined as +/− 100 nt flanking the start site of transcript models).
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