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Average pmch−/− rat gene expression from the three experiments is expressed as a percentage of average pmch+/+ gene expression.
However, validation experiments indicated that the rat gene expression data only increased overfitting during training, so these features were removed.
A recent publication that combined rat gene expression data and a human genetic association study has identified the first genetic risk factor for chronic pain in humans.
Roche Nimblegen Rat Gene Expression array analysis was performed with Affymetrix GeneChip Rat Gene 1.0 ST Array system (Affymetrix, Santa Clara, CA).
All human gene expression was normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA copies, and rat gene expression was normalized to β-actin mRNA copies in all samples.
In addition, rat genome annotation lags behind the mouse and human in maturity, confounding a more comprehensive assessment of rat gene expression data.
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In a study with Sprague-Dawley rats, gene expression was evaluated on days 3 and 11 post-fracture.
In the testes of rats, gene expression of LDHA is down-regulated after phthalate exposure in utero (Plummer et al. 2007) and FGF9 plays distinct roles in testis development and male sex determination (Colvin et al. 2001).
Additionally, seven genes were identified which exhibited the same differential expression pattern in both rat HCA gene expression studies and in livers from rats exposed for 52 weeks to TCDD and/or PCB126, but not PCB153 (Table 6).
Previous studies of rat liver gene expression have also shown age-related changes in xenobiotic metabolism genes, although only male rats were examined, [ 17] and included Cyp2c11 and Cyp3a2.
Statistical analysis of oligonucleotide microarray data was used to identify phencyclidine-induced alterations in rat cortical gene expression.
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