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One rat from each group including control was fixed with 10% buffered formalin following phosphate-buffered saline exsanguinations.
One representative rat from each 6-OHDA-lesioned group except the control was used for TH immunohistochemistry at 12 to 13 weeks after lesioning.
After baseline O2, CO2 and flow were measured, each rat from each group was situated in metabolic monitor cage for 10 minutes and then O2 and CO2 were measured again after the values had stabilized.
One rat from each cage was fear conditioned to a tone CS+ shock US.
Tissue from one rat from each of the four groups was processed concurrently to decrease variation.
One rat from each surgical group was excluded from the experimental protocol as they did not reach the cut-off of 20 s for the initial object exploration.
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Five rats from each group were used for the immunohistochemical examination.
After administration of the formulations, rats from each group were sacrificed after 2, 4, 6 and 24 h.
Some of the rats from each group were also tested for motor stimulation induced by cocaine (5 mg/kg, i.p).
After 1, 3 and 5 days, 10 rats from the control group and 10 rats from each time group of the experimental group were sacrificed, and both left and right kidneys from each rat were removed separately.
Five rats from each group were sacrificed at weeks 4 and 8 post surgery, and a histological analysis was performed on the rats from both groups.
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