The aim of the current study was to find a suitable method of simulating the PSD state in rats for further research into PSD.
Three animals were lost from the normal group during the μCT scanning period due to anaesthesia, leaving nine rats for further analysis.
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Following the activation procedure, embryos were washed at least 10 times and placed in drops of KSOM (for mouse embryos) or mR1ECM (modified rat 1-cell embryo culture medium, for rat embryos) for further development in vitro [24], [34].
Add that to the rat dossier for further study.
Platelet samples were permitted to be taken from anesthetized SD rats (male) for further study.
At the end of each experiment, a blood sample from each rat was collected for further plasma glucose and glycosylated hemoglobin (HbA1c) determination, using the enzymatic method and the turbidimetric immunoinhibition method, respectively (Bangkok RIA Laboratory Co. Ltd ,Bangkok, Thailand).
We excluded this rat from further analysis.
Kinase profiling and structure-based drug design guided the optimization from the initial 1,2,3-benzotriazole hit to a potent and selective JNK inhibitor, compound 24f (JNK1 and 2 IC50 = 16 and 66 nM, respectively), with bioavailability in rats and suitable for further in vivo pharmacological evaluation.
The eye fundi were observed by slit lamp examination at days 4, 14 and 30 after ET. At this time point, one half of the rats were sacrificed for further analysis, and the other half was kept until day 100 for histological analysis.
