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TRPC3 and TRPC6 can form a heteromeric channel complex with TRPC1, TRPC4 and TRPC5 in rat embryonic brain, but not in adult brain [10].
To study cocaine's toxic effects in vitro, we have used primary mesencephalic and striatal cultures from rat embryonic brain.
IGF-1 injection into rat embryonic brain results in a 28% increase in DNA content postnatally as a consequence of increased DNA synthesis and entry into S phase.
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NSCs were derived from Wistar rat embryonic brains, cultured, and expanded.
Bone marrow cells from rats and human embryonic brain cells show a facilitated cell migration [ 153, 154], while bone marrow CD34+ cells have a lower migration potential in simulated microgravity [ 155].
The observations described here derive from experiments designed to identify axonal chemorepellents in which explants of degenerating adult rat brain were co-cultured with embryonic brain explants in collagen gel matrices.
In rats, PDGF-C is expressed predominantly in embryonic brain and spinal cord, while PDGF-D expression is evident in adults rather than in embryos [66].
Its neuronal localization is primarily at the plasma membrane of the soma in the embryonic brain and progresses into homogeneous expression in the postnatal rat brain.
Further, we compared γ-secretase cleavage of endogenous APP, Notch1, N-cadherin, ephrinB, and p75-NTR in membranes prepared from embryonic and adult rat brain and found that cleavage of all of these substrates, i.e. ICD production, was higher in embryonic brain.
We tried the same thing with fruit flies and then with rat embryonic neurons.
Following the discovery that immortalized rat embryonic fibroblasts have circadian rhythms of gene expression [18], real-time monitoring of circadian promoter-driven reporters revealed that peripheral tissues and extra-SCN brain regions exhibit circadian oscillations in vitro [19], [20], [21].
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