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Subsequently, 1 × 106 PBL were subcutaneously or intraperitoneally injected three times into wild-type F344 rats (1 rat each) with an 11-day interval between the injections.
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The hybridization of each organ was repeated 20 times: 4 biological replications (rats), each with 5 technical replications (slides).
In our study the expression pattern of TFAP2 was similar between ZL and ZDF rats, each with highest expression in the hypothalamus and subcutaneous fat (ZL: p < 0.001 for HT vs. KF and MF, p = 0.003 for SF vs. KF, p = 0.021 for SF vs. MF; ZDF: p < 0.001 for HT vs. KF and MF, p < 0.001 for SF vs. KF and MF).
Expressed human CYP3A4, rat CYP3A1, and rat CYP2C11 (each with P450 reductase and cytochome b5), 0.5 M potassium phosphate at pH 7.4, and NADPH regenerating system solutions A and B were purchased from BD Biosciences (Woburn, MA).
Neuropathic pain was surgically induced in 30 male rats by ligation of the L5 and L6 spinal nerves [ 18], with 10 rats each treated with saline and 0.05 and 0.1 mg DBV.
Neuropathic pain was surgically induced in 30 male Sprague Dawley rats by ligation of the L5 and L6 spinal nerves, with 10 rats each treated with saline and 0.05 and 0.1 mg BV.
Recovery of Trichinella after a one-week incubation was sufficient to infect 4 donor rats each inoculated with 300 larvae.
Increase the amount of time that you spend with your rat each day.
SHR were randomly divided into 2 groups (11 rats each): rats treated with esmolol (SHR-E) and rats treated with vehicle (SHR, hypertensive control group).
Estrous cycles were monitored for each rat with immobilization timed to coincide with the onset of estrus, leaving most rats in proestrus 4 days after the end of stress.
Each rat was implanted with a miniature microdrive with two tetrodes aimed at pre- or parasubiculum.
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