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The perifusion of rat cells was performed using low (5.5 mM) or stimulating (8.3 mM and/or 16.7 mM) glucose concentrations or KCl (50 mM) in KRBH buffer-0.05% FAF-BSA, as described previously [13].
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Then, endometrial cell sheets from green-fluorescent-protein rat cells were transplanted onto the buttock muscle of nude rat for evaluating the function in vivo.
Rat cells were more sensitive to COM than HPT cells, but there were no apparent differences between the effects in Wistar cells and F-344 cells.
But Dr. Gorbunova, Dr. Seluanov and their colleagues discovered that naked mole rat cells are much more sensitive to other cells.
These results suggest that HII4E (rat) cells are more protected from oxidative stress than PLHC-1 (fish) cells.
Naked mole rat cells are even more sensitive to their neighbors, the researchers found.
When mammalian (rat) cells were challenged by amino acid starvation, a form of 110S particles, including 80S dimers and 80S-60S heterodimers, were also detected (Krokowski et al., 2011).
This is especially important in xenotransplantation studies, such as the current study where rat cells were transplanted into the guinea pig.
15 20 pluripotent rat cells were injected into the blastocoel cavity.
Rat cells were collected for Western-blot analysis as previously described (Feigenson et al., 2009).
However, rat cells were found to only have contact inhibition regulated by p27Kip1.
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