Your English writing platform
Discover LudwigExact(26)
An efficient mutual information (MI) based automatic registration method is introduced to align the rat brain tissues in magnetic resonance imaging (MRI) time-series, which is critical to the MRI tracer method for quantitative analysis of brain extracellular space (ECS).
The determined physicochemical properties indicate for some of the compounds that they might be suitable for CNS-targeting which was exemplified by the detection of significant baclofen levels in rat brain tissues following an i.p. dose of ethyl carbamate (vs. ethyl ester, for which only traces of baclofen were detected).
Protein lysates were prepared from rat brain tissues or cultured cells for Western blotting analyses using corresponding antibodies following our previously published protocols with a chemiluminescence HRP detection kit (Millipore) (Guo et al., 2011; Deng et al., 2015).
MCT1 and MCT2 were detected by CLSM mainly in the thalamus and hippocampus of rat brain tissues (Figs. 1 3).
The rat brain tissues or mouse primary neurons were fixed in buffered 4% (v/v) formaldehyde for 5 min at room temperature (RT).
Rat brain tissues were lysed in homogenization buffer (20 mM Hepes, pH 7.6, 125 mM NaCl, 10% glycerol, 1 mM EDTA, 1 mM EGTA supplemented with 1% DTT).
Similar(34)
Brain tissue-specific ECM (bECM) was extracted from decellularized rat brain tissue using a protocol previously described (see Methods)12.
Kokubo H, Helms JB, Ohno-Iwashita Y, Shimada Y, Horikoshi Y, Yamaguchi H. Ultrastructural localization of flotillin-1 to cholesterol-rich membrane microdomains, rafts, in rat brain tissue.
Gama, M. A. et al. Conjugated linoleic acid-enriched butter improved memory and up-regulated phospholipase A2 encoding-genes in rat brain tissue.
The isolated ECM from decellularized rat brain tissue has a preferential banding pattern for high molecular weight proteins (Fig. 1b), consistent with previous studies of decellularized rat and porcine brain tissue10,14.
The bECM prepared using an alternative protocol (originally used to prepare decellularized porcine brain tissue14) also exhibited a similar banding pattern to the decellularized protocol for rat brain tissue (data not shown).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com