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NeuN immunostaining of chemically-induced MSC had low intensity compared to neurons from rat brain section (figure 2).
A rat brain section is a standard example tissue in IMS.
Immunofluorescence staining of rat brain section was performed according to the previously described method.
Figure 1A shows an optical image of a transversal rat brain section in the region of the hippocampus.
Results: The proposed segmentation methods are evaluated on two IMS datasets: a rat brain section and a section of a neuroendocrine tumor.
Indirect immunodetection of MC4R in sections of rat brain followed by peroxidase staining, Upper part: control rat brain section, Lower part: negative control without primary antibody (arrowhead indicates the small cells (microglia) positive for MC4R and arrow indicates the large cells (macroglia) positive for MC4R in the brain).
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Application of the new instrument to imaging phospholipids in rat brain sections is described in detail.
Here, we present microwave-assisted staining protocols for floating rat brain sections and cultured rat hippocampal cells.
For rat brain sections, boundaries of partially isolated undercut cortices as well as contralateral homotopic somatosensory cortices were identified as ROI.
In vitro autoradiography using rat brain sections.
In vitro autoradiography using rat brain sections 3.
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