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For morphometric examination, 2-mm-thick 2-mm-thick 2-mm-thick coronalng a rat brain matrix.
Immediately after decapitation, the brains were dissected using a pre-cooled rat brain matrix (ASI Instruments, Inc., Warren, MI, USA).
Brains were dissected, and 1-mm-thick coronal sections were generated using an acrylic rat brain matrix (Electron Microscopy Sciences).
Brains were removed and the whole hypothalamus was dissected using a rat brain matrix [ 37, 38] and frozen in liquid nitrogen and stored at -80°C until processing.
The fixed brain tissue was placed in a coronal rat brain matrix (Activational Systems Inc., Warren, MI, USA) and cut into 2-mm blocks.
The brains were removed rapidly and were placed ventral side up in an ice-chilled rat brain matrix (Harvard Apparatus, Kent, United Kingdom).
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The fixed brain was placed in a 200 400 g coronal rat-brain matrix (Activational Systems Inc., Warren, MI) and cut into 1-mm blocks.
Then, the in vitro biological assays of the conditioned electrospun scaffolds, using rat allogeneic mesenchymal stromal cells, confirmed their biocompatibility and showed a differentiative potential in presence of just 1% w/w decellularized rat brain extracellular matrix.
Brains were obtained and sliced with Rat Brain Slicer Matrix (1.0 mm slice intervals, RBM-4000C, ASI Instruments, Warren, MI) and a razor blade.
Fixed brains were placed on a rat brain slicer matrix and coronal slices containing the frontal cortex were excised and processed for EM.
Using a rat brain slicer (Rodent Brain Matrix, Adult Rat, Coronal Sections, ASI Instruments, USA) 3 mm coronal sections were dissected, cutting at distances of 2, 5, 8, and 11 mm from frontal pole.
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