Sentence examples for rat brain extracts from inspiring English sources
Incubation of rat brain extracts with recombinant CaMKKα (Phe230Gly), but not with wild-type kinase, in the presence of N6- 1-methylbutyl -ATP aN6- 1-methylbutyl -ATPigN6- 1-methylbutyl -ATPsphorylatioN6- 1-methylbutyl -ATPandwell as Ca2+/CaMosphorylatinducedThr177.
Immunoprecipitation of neurabin I and neurabin II/spinophilin from rat brain extracts sedimented PP1gamma1 and PP1alpha but not PP1beta.
However, rat brain extracts depleted of CRAMP have substantially attenuated antimicrobial activity [50].
Having established an acceptable specificity for anti-ΔP antibody preparation, we used it in direct immunoblots of rat brain extracts.
Interestingly, human and rat brain extracts incubated with radiolabeled morphine produce labeled M3G and M6G [22], [22].
In particular, experiments using rat brain extracts preloaded with 45Ca2+ did not reveal a change in Ca2+ efflux over a range of power densities [12].
We observed that unfractionated rat brain extract incubated with substrate at pH 6.0 yielded 12 times the quantity of angiotensin I as incubations at pH 7.4, but the enzyme activity measured at pH 6 was not primarily due to renin.
Analysis of binding of radiolabelled Munc18-1 to assembled SNARE complexes was carried out using a GST-complexin pulldown assay to isolate native SNARE complexes from a rat brain extract as described previously [33].
Since Munc18-1 is also able to bind in an alternative mode to the assembled SNARE complex [12], [13], [15], [32] we also tested the binding of Glu379 in an assay based on pull down of assembled SNARE complexes from a rat brain extract using GST-complexin.
Our own data demonstrated that the increased level of neurotrophic factors in medium following conditioning of murine embryonic stem cells with rat brain extract was due in equal shares to the release of these factors from stem cells as well as from feeder cells [7].
Human MSCs cultured in ischemic rat brain extract also exhibited increased production of BDNF, VEGF, and hepatocyte growth factor [ 64].
