PET images were co-registered with a rat brain atlas for illustration of anatomical regions.
PET images (128 × 128 × 159) were co-registered with CT scans and mapped to a MRI-based rat brain atlas [27].
The unconscious and conscious scan reconstructions were aligned by an affine registration to the Johnson rat brain atlas [31], which was used to delineate regions-of-interest (ROIs).
A magnetic resonance (MR -based rat brain atlas [32] was used to define five regions of interest (ROIs): the hippocaMR -basedipital coratx, parietal cortex, caudate putamen and cerebrainm.
Regions of interest [ROIs] were drawn over the frontal cortex, striatum, locus coeruleus, and cerebellum, which were anatomically identified from the cryomicrotome sections using a rat brain atlas [27].
Images were co-registered manually to the Swanson rat brain atlas [13], smoothed with a Gauss kernel of 1.5 mm FWHM and intensity normalised to the cerebellum (SUVRcer = individual voxel value divided by the mean value of cerebellum).
Images were co-registered manually to the Swanson rat brain atlas [13] and intensity normalised to whole brain activity (standardised uptake value ratio SUVRwb = individual voxel value divided by the mean value of the whole brain).
A stereotaxic frame was designed for animal positioning that could be used in all three measurement modalities, and its dimensions complied with the gold standard of the Paxinos and Watson rat brain atlas.
After exposure of the skull, the coordinates for single-site (1.0 mm anterior and 2.7 mm lateral to bregma) and two-site injections (1.6 mm anterior, 2.2 mm lateral, and 0.4 mm posterior, 4.0 mm lateral to bregma) were determined according to the rat brain atlas of Paxinos and Watson [24].
The striatum was delineated using a 4× objective, in accordance with a rat brain atlas [65].
One section per brain region was matched according to the rat brain atlas of Paxinos and Watson [37] and the neonatal rat brain atlas by Altman and Bayer [38].
