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Cells were purified from infected rat blood using DEAE chromatography, washed and resuspended (107 cells.ml−1) in iso-osmotic PSG (pH 8.0) containing NaCl (44 mM), KCl (5 mM), Na2HPO4 (57 mM), NaH2PO4 (3 mM), glucose (10 mM), sucrose (70 mM).
Bloodstream form cells were purified from whole rat blood using DE-52 anion exchange resin (Whatman) equilibrated to pH 8 with a bicine glucose buffer (50 mM bicine, 50 mM NaCl, 5 mM KCl, 50 mM glucose).
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All five diamidines stained live T. b. brucei in infected rat blood films using a standard fluorescein isothiocyanate (FITC) filter set (excitation, band-pass, 450 to 490 nm; beam splitter, 510 nm; and emission, band-pass, 515 to 565 nm) and a Zeiss Axioplan fluorescence microscope (Fig. 1A).
Regional lymph nodes were removed and cells were separated by mincing the tissue and disaggregating it through a needle of 22 G. PBMNC from naïve rats, AIA rats and IB-MECA-treated rats were fractionated from heparinized blood using the Ficoll Hypaque gradient.
Therefore, we obtained pharmacokinetic data for CN147 (1 mg/kg) in rat brain tissue and in blood using established (invasive and destructive) LC-MS/MS methodology.
BF strain STIB 920 of the same species was used for infection of rats and isolated from their blood using diethyl amino-ethyl cellulose chromatography (Chaudhuri et al. 1995).
This study was designed to study the influence of Sacoglottis gabonensis stem bark extract on the metabolic and cytotoxic side effects of 2,4-dinitrophenyl hydrazine (2,4-DNPH) on the brain and blood using male weaning rats as the experimental model.
Rat blood was used to test the hemolysis effect of DTX-loaded Polysorbate 80/Phospholipid mixed micelles.
Sera were prepared from rat blood and used to determine the contents of ALT, AST, and Collagen I. WM130 and M19 treatments resulted in an obvious decrease of serum ALT, AST, and Collagen I compared with the DMN-induced model control group, but they were also higher than that in the normal control mice).
EK performed the initial analysis using rat blood.
Spontaneous and PMA-stimulated oxidative burst of neutrophils in rat blood were determined by using chemiluminescence.
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