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Elliptical volume of interest was defined around the striatum of an intact rat and used in all subsequent analyses.
To study the mouse lineage, we compared mouse and rat and used human as an outgroup.
The average of a total of three readings was determined for each hind limb for each rat and used for subsequent analyses.
Two hours before sacrifice, blood samples were collected from the tail of each rat and used to determine serum creatine kinase activity.
In the present study, we isolated 4 aortic rings from one rat and used 2 rings as the control group and 2 rings as the experimental group.
Using Bambino (Edmonson et al. 2011), we identified candidate A-to-I RNA editing sites in the brown rat and used Sanger sequencing to validate these from RNA extracted from the cerebral cortex of the same rats.
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On a related note, Kim's colleague Hiromitsu Nakauchi, MD, PhD, recently showed that mouse pancreases could be grown in rats and used to treat diabetes in mice.
To determine whether calcitonin (CT) might effect maturation of mammalian growth plate cartilage, we administered salmon CT (sCT) to young rats and used the growth plate from the distal metatarsal as our in vivo growth plate model.
Mammary glands were removed from age-matched virgin rats and used as controls.
The rats with diabetes having glycosuria and hyperglycemia (blood glucose range of above 150 200 mg/dl) were considered as diabetic rats and used for further experiments.
They took a rat kidney and used a detergent to wash away the old cells.
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