Sentence examples for rat and maintained from inspiring English sources

All rats were housed individually in single cages, to better check food intake of each rat, and maintained under laboratory controlled conditions (22 ± 1°C, 55 60% humidity, 12 h light : 12 h dark).

In one finding, the circadian corticosterone rhythm was entrained by restricted feeding in rats and maintained under enteral injection of artificial nutrition.

Small pulmonary arteries were freshly obtained from rats and maintained in Hank's balanced salt solution (HBSS, Gibco) supplemented with penicillin (100 units/ml, PAN) and streptomycin (100 units/ml, PAN).

Primary cultures of dissociated cortical neurons were prepared from E18 rats and maintained in Neurobasal medium supplemented with 2%B-27B-27.5 mM glutamine, 50 U/ml penicillin, and 50 μg/ml streptomycin (Invitrogen).

Coronary EC were isolated from 250 to 300 g male Wistar rats and maintained in Earle's minimal essential medium 199 supplemented with 10% fetal calf serum and 10% newborn calf serum till two passages as previously described [ 16].

Mosquitoes were blood-fed on anesthetized white rats and maintained under conditions similar to those for sugar-fed mosquitoes, 25°C with 80% humidity and a 12-h light/dark cycle with available 20% sucrose solution, until collection.

They were then blood fed on naïve and P. berghei-infected rats and maintained under similar conditions except that the temperature was lowered to 19 20°C to be permissive for development of P. berghei [ 91].

In contrast, when slices were prepared from morphine-pre-treated rats and maintained in this concentration of morphine, profound MOPr desensitization was observed that could be reversed by inhibiting PKC.

Dorsal root ganglia (DRG) cultures were produced from E16 to E17 Sprague Dawley rats and maintained as described previously with minor modifications [ 37]. (See Online Resource 1: Supplemental Methods for additional details).

Neonatal rat cardiomyocytes (NRC) were isolated from neonatal rat ventricles and maintained as previously described (Lange et al., 2002).

Cell Culture and Immunofluorescence Neonatal rat cardiomyocytes (NRC) were isolated from neonatal rat ventricles and maintained as previously described (Lange et al., 2002 ).