Sentence examples for rare templates from inspiring English sources

Therefore, some rare templates may be amplified in sorted samples and not in filtered samples, because template competition is lower in the former samples.

This feature and the fact that single-stranded amplicons do not engage the polymerase ensure efficient amplification of abundant as well as rare templates in a sample.

In order to amplify even very rare templates, 1 μl samples of the above PCR reactions (total new reaction volume was 100 μl) were subjected to a second 35-cycle round of PCR, and the products were resolved by agraose gel electrophoresis.

However, we found out that TaqMan® Low Density Arrays are typically not the method of choice for the detection of rare template molecules.

To explain the etiology of prion diseases, new mechanism proposed that in animals the transition from rPrP fibrils to PrPSc consists of two main steps: the first involves fibril-induced formation of atypical PrPres, a self-replicating but clinically silent state, and the second consists of atypical PrPres-dependent formation of PrPSc via rare deformed templating events.

TR should not be better than GDT_TS either for models based on close homology where model target misalignments are rare, or for template-free models where even a general fold prediction is a challenge.

Besides, the additional rare mutation in the template can even introduce false negative test result during the allele specific PCR assay [ 12].

SWIFT and its adjunct programs have been optimized to detect very rare populations, and the templating strategy allows stringent comparisons between large numbers of samples.

Finally, we demonstrate that our method makes it possible to use external imputation templates to analyze rare variants imputed into existing GWAS datasets.

High-throughput cloning of cDNAs requires relatively high template quantities because rare transcripts should be amplifiable.

Most template switching events are rare but the huge volume of transcriptome data currently being produced ensures that their contamination of cDNA databases will increase.