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This allows donor HSC to effectively compete for space in the BM HSC niche in order to rapidly reconstitute the hematolymphoid system.
This implies that reactivation of B lymphopoiesis does not occur rapidly to allow expansion of lymphoid progenitors, thereby explaining the failure of BM cells from old mice to rapidly reconstitute the B lineage in BM chimera or after lymphoablation [ 26, 60].
As seen in Figure 3 and Supplementary Figure 3, as few as 50 100 SP cells from H146 and H526 cells, respectively, were sufficient to rapidly reconstitute the tumours, whereas mice that received 50 100 non-SP cells did not develop tumours, or had only palpable tumours in the same time frame (less than 5 mm).
During tissue repair, the body acts in a series of tightly regulated steps to rapidly reconstitute damaged tissue: the formation of a fibrin clot acts as the platform for granulation tissue assembly, with subsequent contraction and remodeling of the ECM to reform normal tissue [ 1, 2].
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Morels are easily preserved by drying and can be rapidly reconstituted in warm water; the resulting taste and texture are every bit as good as with fresh ones.
Lyophilized samples were rapidly reconstituted using high purity water.
Furthermore, as shown in this report, functional IFP can be easily and rapidly reconstituted after in vitro transcription/translation. Using the LIC-compatible vectors provided here, also untagged proteins can be produced.
Upon restoration of isotonic conditions the polysomal fraction is rapidly reconstituted.
In contrast, as few as 50 SP cells from H146 and H526 SCLC cell lines rapidly reconstituted tumours.
Concomitant with the depletion of ATR-deleted cells, most of these same tissues were rapidly reconstituted with the small percentage of ATR-expressing cells that escaped lox recombination.
The free intracellular alanine pool was rapidly reconstituted by extracellular 15N-alanine, an effect markedly inhibited by MeAIB, with little contribution from unlabeled alanine or alanine generated from 13C-glucose-derived pyruvate.
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