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Our observations indicate that the effects of RNA degradation following death or tissue isolation are pervasive and can rapidly obscure inter-individual differences in gene expression.
Relatively mild fragmentation will thus rapidly obscure the rRNA bands when visualized by electrophoresis, and drop the RIN values on the Bioanalyzer instrument (Agilent) into the 3-5 range.
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Under normal circumstances, these initial interactions are rapidly obscured by the more robust Ndc80 complex-dependent attachments of kinetochores to microtubules.
For Nes-Cre Wt1co/co kidneys, we could use the brightfield signal but, in control kidneys, the development of nephrons rapidly obscured the ureteric bud, so for this we used Hoxb7-Cre (Yu et al., 2002) to activate the eYFP reporter specifically in the ureteric bud and analyzed the fluorescent signal.
"It is a very dangerous stretch of the beach," said Cal Fire Battalion Chief Jake Hess, who explained that the tide changes rapidly and obscures any pathway to shore.
In the struggle for advantage in the digital advertising boom, companies like Google, Yahoo, Microsoft and AOL are rapidly acquiring once-obscure firms, sometimes for eye-popping prices.
However, intratracheal instillation of saline can also be criticized because a proportion of the fluid is rapidly absorbed and obscured from detection [ 34].
He added, "That class of data, that's technically public but really obscure, is rapidly diminishing".
It even comes with the promise of returning London to the hazy world it was when the misty-eyed Mr Turner roamed the banks of the Thames, complete with a Battersea Power Station merrily chugging out enough smoke to obscure the towers rapidly rising along the riverside.
The positioning errors grow rapidly when obstacles or terrain obscure the sky precluding GPS receivers from obtaining signals through a sufficient number of satellites.
We assessed, in a subject with a selective loss of large diameter myelinated afferents, cortical responses to high-intensity electrical and laser stimulation of small-diameter afferents; such input is in control subjects obscured by more rapidly conducting Aβ-input.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com