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Either marker appears equally effective, but there is considerable merit in using a pyrE mutant as the host because, through the use of ACE vectors, mutants created in such strains (by whatever means) can be rapidly complemented.
More importantly, mutants created in pyrE strains (by whatever means) can be rapidly complemented using ACE vectors by the introduction of a functional copy of the inactivated gene concomitant with restoration of the pyrE allele to wild-type.
The codA or pyrE counter selection markers appear equally effective in isolating deletion mutants, but there is considerable merit in using a pyrE mutant as the host as, through the use of ACE (Allele-Coupled Exchange) vectors, mutants created (by whatever means) can be rapidly complemented concomitant with restoration of the pyrE allele.
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As these genes had not been previously inactivated, they provided the opportunity to make use of one of the key advantages of the pyrE system, the ability to rapidly complement mutations at an appropriate gene dosage in the genome.
Their isolation provided the opportunity to make use of one of the key pyrE system advantages, the ability to rapidly complement mutations at appropriate gene dosages in the genome.
This project adds to that toolbox by introducing the Streambed Point Velocity Probe (SBPVP), a reusable tool designed to quantify groundwater-surface water interactions (GWSWI) at the interface with high density sampling, which can effectively, rapidly, and accurately complement conventional methods.
The dynamic research environment at GenProfile is complemented by a rapidly improving environment for bioinformatics in Berlin.
Neuroimaging has profoundly advanced neuroscience research and clinical care rapidly in the past two decades, prominently by magnetic resonance imaging (MRI), complemented positron emission tomography (PET), and electroencephalography (EEG /magnetoencephalography (MEG).
The principles of "Moneyball" — celebrated in a popular book and movie — spread rapidly, with objective analysis complementing traditional scouting to form a better understanding of talent and value.
These rapidly evolving therapies may complement and enhance other mechanical and surgical interventions for patients with congestive heart failure, providing cardiac surgeons with a wider range of treatments for patients at risk of congestive heart failure.
Complementing a rapidly evolving locus such as the trnH-psbA spacer with a more conservative locus (such as the coding locus rbcL) can minimize type I errors (such that sequences are robustly assigned to the correct genus at least) and type II errors (higher rates of sequence divergence can discriminate among closely allied species in highly speciose genera).
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