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In brief, intestinal epithelial lymphocytes were separated from intestinal pieces by incubating 1-cm2 pieces of tissue in EDTA with rapid shaking at 37°C.
After epithelial removal, LPL were collected by mincing the remaining tissue into 1 2 mm pieces, followed by digestion in complete RPMI 1640 medium (RPMI-5) containing 60 units/ml of Type II collagenase (Sigma-Aldrich) again with rapid shaking at 37°C.
Since we did not have proper equipment to run aerosolation experiments under varied humidity, we tested the stability of Lula and Lambda to surface tension via rapid shaking in liquid, which introduces multiple small bubbles in the phage suspension and is generally compatible with aerosolation in its killing effect on phages [22], [25], [26].
Each filter was extracted by rapid shaking with 4 mL of endotoxin-free water for 1.5 h.
Briefly, biopsy was dissected and digested for 1 2 h with collagenase A (0.33 U/ml), dispase (0.85 U/ml) and Dnase I (144 U/ml) with rapid shaking at 37°C.
Freshly thawed FBS (Gibco) was heat-treated at 75 °C for 0.25, 0.5. 1, 2, 5, and 10 min in 1 mL aliquots on an Eppendorf thermoshaker with rapid shaking.
Similar(48)
This effectively crippled the stingray, which was then picked up in the jaws and sawed apart with rapid shakes of the head.
Beta blockers, such as atenolol (Tenormin) and propranolol (Hemangeol, Inderal), can control rapid heartbeat, shaking, and trembling in anxious situations.
Previous studies indicate that inadequate emergency response can increase the number of casualties by a maximum factor of 10, which suggests the need for research on rapid earthquake shaking damage and loss estimation.
Do you get any of these signs: sweaty palms, heart pounding, jaw clenched, upset stomach, headache, clenched fists, rapid breathing, shaking, urge to drink or use drugs, tunnel vision, muscle tension, urge to smoke, facial distortions, butterflies, lump in throat, tight chest.
Three explosions in rapid succession shook central Baghdad in what was apparently a mortar attack on the headquarters of the American civilian authorities.
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