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The strategy offers a new pathway for rapid RAC analysis and shows a promising prospect in food safety.
A working model for force-induced rapid Rac activation is illustrated in Fig. 7B.
Ten nM R1881 triggers rapid Rac 1 activation in parallel with cytoskeleton changes in NIH3T3 fibroblasts [10].
Our finding on the stress-induced rapid Rac activation challenges the conventional wisdom on mechanotransduction and suggests that stress-induced signaling does not follow signal transduction pathways induced by growth factors.
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Again, this is too slow to explain the rapid activation of Rac that we have observed in living cells.
Cells attached to the dishes were collected and referred to as rapid adherent cells (RAC).
f = CCM Community case management, RAC Rapid assessment and care coordination.
Contrary to the current working model, rapid activation of endogenous Rac proteins triggered effective actin polymerization but failed to feed back to PI3K to generate PIP3 or induce cell polarization.
Yet, the rapid endosomal transport of Rac and Cdc42 on early endosomes also suggests the existence of an 'active performance' model, where (most frequently) early endosomes deliver signaling components very quickly and with high accuracy.
Mercury intrusion porosimetry was used to test the pore structure of the mortar in RAC, and the rapid chloride migration instrument was selected to investigate the diffusivity of RAC.
Markedly, synthetic activation of endogenous Rac led to rapid and symmetric pseudopod formation and ruffling along the cell periphery, but did not result in cell polarization or significant migration (Fig. 2B, Movie S2).
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