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Liu, Y. G. & Whittier, R. F. Rapid preparation of megabase plant DNA from nuclei in agarose plugs and microbeads.
This pointcut of rapid preparation of globotriose derivatives is proposed for the first time.
Our highly adaptable self-assembly approach enables the rapid preparation of ranges of water-stable, helicate-like compounds with high stereochemical purity.
MIPs are often called synthetic antibodies in comparison with immuno-based sorbents; they offer some advantages including easy, cheap and rapid preparation and high thermal and chemical stability.
Previously we reported a method called DelsGate for rapid preparation of deletion constructs for protoplast-mediated fungal transformation systems, which is based on Gateway® technology.
Co-immobilization of cell adhesion peptides and growth factors on thermoresponsive surfaces should be effective for rapid preparation of intact cell sheets and their utilization to regenerative medicine.
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BM aspirate (30 mL) was collected during 3 orthopedic surgical procedures, and a concentration system was used to achieve 3 rapid preparations of a concentrate of nucleated cells (3 mL) from the BM aspirates.
To look at the cellular organization of roots, 5-day-old roots were hand-sectioned according to the protocol "Rapid preparations of transverse sections of plant roots" (http://www.mcdb.lsa.umich.edu/labs/schiefel/protocols.html).html
Sequencing libraries were prepared following the Rapid Library Preparation Method Manual (Roche, GS FLX Titanium series) starting from 1 μg of the ligated ds cDNAs.
Roche 454 libraries were prepared following the 'Rapid Library Preparation' method according to manufacturer's recommendations for single-end pyrosequencing using the Roche 454 GS FLX System and Titanium XLR70+ kit (Roche 454).
For each sample, a cDNA library was prepared with mRNA or cRNA using a cDNA rapid library preparation kit (Roche, Branford, CT, USA) according to the manufacturer's instructions, with minor changes.
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