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Conversely, when rapid dilution of the denaturant was most efficient, a combination of batch dilution and ultrafiltration was recommended.
This involved rapid dilution of unfolded OmpC into a refolding buffer containing polyoxyethylene-9-lauryl ether (C12E9) and glycerol.
Particle samples were withdrawn from the centerline of the combustor through 10 sampling ports by a rapid dilution sampling probe.
The solution is to design marine outfall for rapid dilution to reduce salinity down to safe level.
This was an indication that rapid dilution of the discharge resulted in a negligible effect on ambient salinity and temperature profiles.
Results suggested that the effect of transformation processes was nearly complete within about 1 s after emission due to rapid dilution in the vehicle wake.
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Generated particles are sampled using rapid-dilution probes and the size distribution functions are measured on-line by a high resolution Differential Mobility Analyzer.
Fixer: Tetenal Superfix plus rapid fixer, dilution 1 4.
Fixer: Tetenal Superfix plus rapid fixer, dilution 1 4 Wash: washing in running tap water for 15 min.
Similarly, chemical signaling in 3D suffers from rapid dispersive dilution, thus the diffusion-mediated 3D intercellular correlations are much weaker compared to the case in 2D (Sun et al. 2012).
The denatured protein was refolded by a two-step protocol, the first step being a rapid fivefold dilution of the protein solution in 6 M GuHCl in ice-cold water.
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