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With PIPAAm PEG -PMs, grafted PIPAAm PEG -PMs,d accelerate the diffusion of water molecules to PIPAAm grafted showing more raPEG detachains of cell shouldcompaccelerateAAm-PMs.
Both electrochemically-induced local pH lowering and global decrease of the environmental pH allowed for a rapid detachment of intact stem cell sheets.
Adding a low concentration of nontoxic ferrocyanide to the cell culture medium resulted in erosion of the polyelectrolyte multilayer and rapid detachment of viable cell sheets.
The retained pNIPAAm films (20 40 nm) exhibited thermo-responsive behavior, determined by water contact angles and film thickness in water at temperatures above and below the lower critical solution temperature of pNIPAAm, as well as good cell attachment and rapid detachment (<10 min).
All connections between the HMDS and the cockpit were designed for rapid detachment, for safe use of the ejection system.
The results show that HAMLET triggers rapid detachment of tumor cells, but not of normal differentiated cells.
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However, when the graft densities of the upper PIPAAm and basal PAAm were optimized, the resulting temperature-responsive cell culture surface with the double polymeric layer exhibited rapid cell detachment while maintaining cell adhesive character comparable to that of PIPAAm-TCPS.
Acute exposure to hypoxia of cells at confluence caused rapid cell detachment.
Instead, multiple MAGUKs are probably cleaved in parallel to ensure a really rapid cellular detachment.
Upon lowering temperature, the grafted polymer was speculated to accelerate the hydration of modified PIPAAm gel, resulting in a rapid cell sheet detachment.
By lowering temperature, a rapid cell sheet detachment within 15 min was observed from the surface of the semi-IPN NC gel containing 0.2 w/v% of AL without trypsin treatment.
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