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A transcript obtained in a given library showed the highest expression in the expected tissue, as evidenced by checking the result with rapid PCR validation (Fig. 2) and from several individuals by quantitative PCR (Fig. 3).
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Experimental PCR validation of CNV predictions.
Gene alignments for homeolog-specific PCR validation, along with a summary table are available at https://doi.org/10.5061/dryad.org/10.5061/dryad
Reverse-transcribed PCR validation of the C. cladosporioides MD2 unigenes.
Microarray differential expression by real-time PCR validation of eight extracellular matrix-associated gene transcripts was confirmed.
However the RNA from Suraksha was used for real-time PCR validation.
Real-time PCR validation of differentially expressed genes.
Figure S2 PCR validation of cloned hCNTF in pOPIN vectors.
Real-time PCR validation of RNA-seq results.
HWP was responsible for PCR validation of miRNA.
PCR validation is described below and in the Supplementary Methods.
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