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RAP buffer was used for tissue lysis.
Similar(59)
Atlantic relied on a formula: radio-friendly raps buffered by giant sung hooks.
The RNA was eluted into 150 μl elution buffer, and stored at -80°C RAP-PCR was carried out according to the method of Judex et al. with some modifications [ 18].
Competitive inhibition of [I]-Angiopep-2 uptake was performed with receptor-associated protein (RAP) (40 μg ml−1 or 1 μ) with a prewash of 1 h in the Ringer/HEPES buffer to clean surface receptor of the cells.
Cells were rinsed twice with cold PBS and then incubated in 0.5 ml ice-cold ligand binding buffer (DMEM-5 mM CaCl2 containing 0.6% bovine serum albumin) with 5 nM 125I-RAP.
The final RAP sample, with a purity greater than 95% as verified by SDS/PAGE, was prepared by dialysis into the assay buffer (20 mM Tris/HCl, pH 7.4, 150 mM NaCl and 1 mM CaCl2).
Rap, rap!
RAP RULES -- Rap, rap.
I rap.
She the buffer.
Identify a buffer zone.
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