Sentence examples for rap amplified from inspiring English sources

Again, we found that PCR duplicates in Rap amplified long-insert libraries were high in both replicates.

The first observation is that the Rap amplified libraries produce the worst assembly, and as expected the most contiguous and accurate assembly is produced from unamplified library.

However, Rap amplified libraries have shown lower number of wrongly orientated reads, suggesting an alternative mechanism that attributes to biases to different genome features.

Constructing a good Illumina library from Rap amplified products seemed to be more difficult than Genomiphi based protocols, considering the lower number of mapped reads and higher number of PCR duplicates.

The assembly scaffolded with the Rap amplified library, which contains the fewest wrongly oriented reads in among all amplified libraries but has the most PCR duplicates, shows the fewest additional miss-assemblies and with both the N50 and longest scaffold length very similar to the unamplified sample.

Seven different rap-1a genes were amplified in Babesia sp. Xinjiang, using degenerate primers designed from conserved motifs.

A total of 25 bands were found differentially amplified by RAP-PCR.

From each of these genes, cDNA was amplified using RAP-PCR as described recently [ 10, 11].

None of the genes, which were found differentially amplified by RAP-PCR fingerprint in the laser dissected ileal PP follicles, were verified as significant regulated (P < 0.05) by real-time PCR.

On the other hand, libraries amplified using Rapisome (Rap) showed different patterns to Genomiphi based protocols.

For phylogenetic study, genomic DNA of randomly selected field samples was amplified with Group II nested PCR primers (Table  1) targeting gp45 and rap-1 fragments of B. bigemina and B. bovis, respectively.