Because most vectors have limited host ranges, vector-mediated HGT from Brassicaceae (or from Fabaceae, in the case of albumin 1[ 35]) to Orobanchaceae and Convolvulaceae appears to be unlikely.
Sindbis virus: An efficient, broad host range vector for gene expression in animal cells.
A genetic construction was carried out using the broad host range vector pKT230 and plasmid pMCG898, which encodes the Erwinia herbicola pyrroloquinoline quinone (PQQ) synthase, a gene involved in mineral phosphate solubilization (mps).
Plasmid pRK404 a smaller derivative of RK2 is a tetracycline-resistant broad-host-range vector that carries a multiple cloning site and the lacZ peptide that enables blue/white selection for cloned inserts in Escherichia coli.
Conclusion: The broad-host-range vector for marker-free allelic exchange described here, pCM433, has the advantages of a high copy, general Escherichia coli replicon for easy cloning, an IncP oriT enabling conjugal transfer, an extensive set of restriction sites in its polylinker, three antibiotic markers, and sacB (encoding levansucrase) for negative selection upon sucrose plates.
Source of tetracycline promoter (PTet) (Ishiwa and Shibahara 1985) pQF50 A broad-host range vector.
All plasmids for overexpression of phaZ genes were derived from the broad host range vector pBBR1MCS-2 (Table 2).
The slant-range vector from the aft antenna phase center to the target can, therefore, be expressed as. (36a).
As discussed in Materials and Methods, the broad host-range vector pBBR1MCS-2 was used as a parental plasmid to construct the phaZ overexpression plasmids.
pneumoniae T7 1,3-propanediol producing strain Laboratory's stock Plasmids pHA10 Ampr, expression vector Arai et al. (1991) pHA12 Ampr, expression vector Arai et al. (1991) pQF50 Ampr, broad-host-range vector Farinha and Kropinski (1990) pHA10-lacZ Ampr, pHA10 containing lacZ This study pHA12-dhaB Ampr, pHA12 containing K. pneumoniae dhaB This study.
The amplified DNA fragment was then cloned into the broad-host-range vector pBBR1MCS-5 [25] to generate the recombinant plasmid pBBR1/katE.
