Sentence examples for ranges of amplification from inspiring English sources

Thresholds were manually set to determine CT values for each sample using the Opticon Monitor 3 software (BioRad), and linear ranges of amplification were determined using LinRegPCR software [55].

The real-time PCR assay, based on the fluorescent double-stranded DNA binding dye SYBR green, generated approximately a 4-log linear range of amplification and in the range of 105 108 (CFU/ml), the coefficient of variation<5%.

Titrations were performed to ensure a linear range of amplification.

Values used were collected from the linear range of amplification.

For all PCR studies, the number of cycles employed was within the linear range of amplification.

Titrations were performed to ensure that PCR were carried out in the linear range of amplification.

Data was analyzed with a threshold set in the linear range of amplification.

Quantifications were performed by positioning the cycle threshold within the linear range of amplification curve.

Data were analyzed with a threshold set in the linear range of amplification.

PCR was done in the linear range of amplification (determined for each primer pair-cDNA combination).

Cycle numbers varied depending on the primers used and they were all within the linear range of amplification.