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Values expressed as measurement errors were converted to ranges and all temperatures were converted to degrees Celsius.
Clearly, both stages of MetaPSICOV significantly outperform the other methods at both sequence separation ranges and all four contact subsets.
The loci were arranged in multiplex PCR panels with non-overlapping size ranges, and all amplifications were carried out using the Qiagen Multiplex PCR Kit (Qiagen) containing 1× Qiagen Multiplex PCR Master Mix, 0.5× Q-Solution, 2 ρmol of each primer, 10 20 ng of template DNA and MQ water for a final reaction volume of 10 μl.
Similar results were found for multilocus genotype diversity (DG), which ranged from close to zero (population C) to 0.794 (population H), with a mean value of 0.215 (Table 2).> PCR products were successfully obtained from all examined individuals, their fragment lengths fit into the predicted size ranges, and all examined loci were polymorphic across the nine populations.
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