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Descriptive statistics (mean and accompanying 95% confidence intervals, median and corresponding inter-quartile range) were quantified using Sigma Stat v. 3.5.
Three subsamples of the plasmid diluted to the optimum concentration range were quantified using ddPCR.
Films were scanned and signals in the linear range were quantified using DigiDoc software (Alpha Innotech, San Leandro, CA, USA) and normalized to β-actin levels.
Concentrations in samples with luminescence above this range were quantified using four points from the linear portion of the dose response curve (log10[estradiol] vs. mean luminescence; 1.2 × 10 10 to 1.9 × 10 9 M estradiol), extrapolated from these standards, and reported as ng/POCIS estradiol equivalents (E2Eq).
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Images were quantified using Image J software.
Muscle and adipose tissue volume and composition were quantified using standard Hounsfield Unit ranges.
Formazan concentrations were quantified using an ELISA reader at 450 nm (reference range 420-480 nm).
Immunoblot signals were quantified using the Odyssey software, and a linear response was confirmed over a 32-fold range.
Data were quantified using MetaXpress software.
All images were quantified using ImageJ (NIH).
NRF2 images were quantified using ImageJ.
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CEO of Professional Science Editing for Scientists @ prosciediting.com