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New primers were designed within conserved genome regions to optimize the detection range of virus subtypes of each genus.
The capacity of the multiplex RT-qPCR assay in detecting the three vitiviruses in mixed infections from a range of virus concentrations in the host was assessed.
Shared international databases of whole ecosystem interactions across a wide range of virus patho- and symbiotic-systems will form the basis for making step-change advances towards our collective ability to engineer and improve mild strain cross-protection.
Five sets of novel genus-specific primers were designed and tested in reverse-transcription polymerase chain reaction assays against a range of virus isolates in plant virus diagnostic laboratories in both Australia and New Zealand.
This assay can tolerate a wide range of virus input but is more sensitive to cell concentration.
The resulting panels represent the diversity of neutralization profiles throughout the range of virus sensitivities identified in the original panel of 200 viruses.
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The technique could aid the development of new vaccines against a range of viruses, including Dengue and Zika.
Some of the compounds display activity similar to the parent nucleoside against a range of viruses.
"We think there may be a huge range of viruses involved," said Mady Hornig, an epidemiologist at the Center for Infection and Immunity.
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