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This was done to visualize better the full dynamic range of the read coverage.
The log2 of the frequency of the reads was plotted to better visualize the extensive range of the read coverage.
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During this process a median of 20% (range 9% 37%) of the reads were discarded from each sample so that a median of 16,016 (range 2,406 35,401) reads remained from each sample.
The known miRNAs (344 precursors, 247 mature miRBase Release 19) comprised a wide range of the mapped reads (16 62 %), with the brain samples having the maximum amount (38 62 %) and with the ovary and testis having the minimum amount (6 8 %).
The step size of the read range was experimentally determined to be around 50 cm.
I wanted to do this column as I've read the Guardian since primary school and am keen to show that a wide range of people read the newspaper – not just the stereotypes.
The range of read depths along with read-depth uniformity of variant calls varied between pipelines (see Additional file 1, Figure S3).
Again, in defining the mean m and σ1, we should take into account the large range of different read count magnitudes; thus they are defined separately for each of the genes.
In fact, while the first versions of the Illumina Genome analyzer had sequencing capacities in the range of several millions reads, the latest Hiseq2000/2500 versions provides more than 3 billion reads per flow cell.
The wide range of read length was applied to include the ribosomal footprints that might be over or underdigested.
It supports adapter detection and removal, demultiplexing of barcodes, paired-end data, a range of read architectures and the efficient removal of sequence redundancy.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com