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In general, samples were diluted 1 to 5 for sFlt1 and sEng, and 1 to 2 for PlGF in order for the samples to fall within the measurable range of the kit's standard curves.
The measurable range of the kit is 0.01-4.0 mM.
The assay range of the kit was 10 2000 mAU/ml.
Where concentrations were above the detection range of the kit, samples were diluted according to the manufacturers' instructions to obtain a value within the detectable range, which was then multiplied by the dilution factor to obtain the final concentration.
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Plasma samples were diluted until the LBP concentration was in the range of this kit.
The detection range of this kit was 0.1EU/ml to 1.0EU/ml.
In addition all samples obtained from both normals and pathologics regarding bone densitometric evaluation were evaluated with the same method, without prior purification, and all obtained measurements were within the detection range of this kit (0 250 pg/ml).
The ranges of the kits for sCD95 and sCD95L were 0.23 15 ng/mL and 0.72 12.92 ng/mL, respectively.
The purification was far more efficient than would be predicted for the methodology and capture-probe range of the Microbe Express kit (Ambion) used for the subtractive hybridisation of rRNA.
Most of the plasma concentrations measured were below the quoted range of the ELISA kit (9 pg ml−1).
The detection range of the assay kits of serum 1CTP was 0.156-10 ng/mL, and the minimum detectable dose of serum 1CTP was less than 0.03 ng/mL.
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