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However, there is a lack of reliable data covering the actual range of residue load in the plants [79].
This same approach was presented earlier for a limited range of residue groups in milk [ 34] and meat [ 35].
Our observations that TM residues tend to be more conserved than EM residues both globally (fig. 2) and across the range of residue burial (fig. 4) are based on averages over 59 yeast membrane proteins.
A similar effect is seen for I97W, as reported above; these residues are expected to be separated by ∼9 Å (measured Cβ Cβ distance) in the range of residue separation in the closed pore.
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The pectoral muscles (n = 128) were assigned to 4 balanced groups according to the number of whole Pb shot and shot fragments recorded (with each group having a suite of samples containing the full range of residues).
Approximately 67% of the clones from the sorted library 3 actually contained the peptide, ENLYFQG, whereas the rest exhibited quite a diverse range of residues occupying the randomized positions.
HLA-B*35-Py molecules preferentially bind peptides with a proline (P) at anchor residue 2 and a tyrosine (Y) at position 9. HLA-B*35-Px molecules also bind peptides with a proline at anchor residue 2, but can accept a much broader range of residues at position 9.
Taken together, our results considerably narrow the prospective range of residues necessary for a stable NOD2/NLRP1 interaction by demonstrating that the constructs NOD2-S (1 180) and NLRP1 linker (92 341) are still capable of an interaction, while further truncated versions of NOD2 are not.
The irrE mutants obtained in this study were sequenced, and the mutations are summarized at both the primary sequence level (for all mutations), and at the homology model level (for mutations within the range of residues 44 303), shown in Figure 9A and Figure 9B.
The range of residues required for TLR5 activation (Andersen-Nissen et al., 2005 ) are indicated by the top bracket.
Peptide binding to CLR/RAMP3 involved a more restricted range of residues than that to CLR/RAMP1 or CLR/RAMP2.
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